How do I install a cDNA/EST/mRNA search database?
GrailEXP expects to search a LIST of databases. The list file can contain one database or many databases. It allows the user to maintain multiple databases (i.e. keeping human and mouse separate, or keeping complete cDNAs separate from ESTs, etc.) but search them with a single list file. The list file should simply contain the full pathname to each search database, one per line. Some preformatted databases are available at the ORNL web site. Each database in the list should be in the GrailEXP Database Format (GXPDF), an explanation of which follows in the next section. There are many issues to resolve before installing the database. Do you want to partition the database into pieces or maintain it as one huge database? Do you want to keep mouse separate from human or put them in a single database? Do you want to put computational assemblies in with EST fragments? All these issues must be resolved before you can build your database. By default, GrailEXP looks for the list of search datab