How do I find the most significant expression changes between classes of gels?
Melanie offers several possibilities to carry out statistical comparisons between populations of gels. You can find significant protein expression differences based on the probabilities from the ANOVA test, available in the Class Analysis Table. You can also use the Overlap values that are displayed in the Class Analysis Table. First, we recommend to filter the spots according to their Ratio (for instance, you can keep the spots that have a Ratio higher than 2), and then to sort the remaining spots according to their Gap values. Generally, this method gives very good results. Please note that this procedure is illustrated in the Quick Start Manual (QSM).
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