How do I figure out where the vector insertion has occurred within my gene of interest to make the best choice of the clones available?
There are several different web based programs that can help you to determine this. Here is a short tutorial on how to utilize Ensembl for this purpose. Ensembl Viewing of Clones One way to visualize gene trap insertions is to go to the Ensembl browser and find your gene of interest, either by blasting a sequence (http://www.ensembl.org/Multi/blastview) or looking for the gene ID in the mouse database (http://www.ensembl.org/Mus_musculus/index.html). Once you have your gene selected, it will usually be as a GeneView window, look at the information within the genomic location section and click on the link at the end of “the start of this gene is located in “Contig …”. Once in the ContigView, you will see a section labelled Detailed view with various pull down menus located across the top. This allows you to customize the browser. Click on the DAS Sources (for “Distributed Sequence Annotation System”) you will see the “Gene Trap” as one of the selections. Check this box and then select “
