How do I ensure optimum aspiration with less than the full 10 µL volume?
Always leave the pipettor set to 10 µL. A full 10 µL volume is recommended for all solutions throughout the OMIX procedure EXCEPT for the final elution volume when lower quantities are required for peptide enrichment. Optimum recoveries during the final step are obtained when the elution reagent is pipetted into a separate vial or well plate. Then, with the pipettor set to 10 µL, carefully pipette the entire volume of elution solvent into the OMIX tip. It is not recommended to aspirate and dispense the sample more than once during the elution step.
