How do I elute my target protein from biotinylated DNA?
• The most common methods are to use either a high salt buffer or boil the Dynabeads® with DNA-protein complex in SDS sample buffer for 3-4 minutes. With a high salt buffer, a salt concentration higher than 1 M is normally applied to break DNA-protein interaction. The exact amount of salt required depends on the affinity of the protein for oligonucleotides and should be determined for each application.
