How do I eliminate the band broadening I get on subsequent runs when working with high concentrations of proteins?
[top] Use a Water Rinse. It is recommended that when your samples are high concentrations of protein or simply a very sticky protein, that immediately before you inject the sample, between runs, that you dip your capillary in CEwater. It is further recommended that immediately after the separation, that you dip your capillary in CEwater. This will assist in removing any protein that will stick to the outside of the capillary walls and will be injected as a type of sample carry over. Also, it is imperative that between runs, that you follow the water rinse with injection of .1N NaOH according the CElixir Operating and Trouble Shooting Manual.
