How do I detect coliforms and E.coli in RABIT?
DETECTION OF COLIFORMS AND ESCHERICHIA COLI IN RABIT Introduction MacConkey Broth is used for the detection of coliforms and E.coli in the RABIT system by the direct impedance technique. Automatic enumeration of coliforms can be achieved by calibrating RABIT results against VRBA pour plates, but it must be noted that RABIT detection of E.coli is on a presumptive basis only. Preparation 1. Whitley MacConkey Broth is prepared from the dehydrated base at a concentration of 35.0g/l. Sodium deoxycholate must also be added at a level of 0.5g/l to enhance selectivity for the target organisms. Disperse the weighed powders in the appropriate quantity of deionized water. 2. Mix to completely dissolve all the constituents. 3. Dispense the broth into its final containers for autoclaving. If preferred, dispense directly into RABIT cells. 4.
