How do I design a genotyping protocol for mice made from mutant ES cells?
The CMMR validates sequence tags by either RT-PCR, 3’-RACE, inverse PCR (iPCR) or splinkerette (SPLK) PCR. Protocols for these can be found with our other SOPs. The CMHD Gene Trap Core has a very good description of the steps involved in designing genotyping protocols for clones whose sequence tag was generated using 3’-RACE on their How To web page.
