How do I correlate data from cDNA-expressed microsomes to data from human liver microsomes?
In vitro studies requiring a quantitative comparison of cDNA expressed microsomes to human liver samples must be based on the cytochrome P450 quantity and activity in each microsome source. The method using relative activity factors (RAF) is summarized in the following papers: “Use of cDNA-Expressed Human Cytochrome P450 Enzymes to Study Potential Drug-Drug Interactions,” Crespi, CL and BW Penman, (1997) Adv. Pharmacol. 43, 171-188.
