How do I clean the Typhoon Variable Mode Imager platen and sample press?
The scanning of gels (especially naked gels) and blots containing stains or other fluorescent compounds may leave fluorescent residues on the platen and/or sample press. These may show up in subsequent scans, obscuring or altering the intensity/profile of protein spots in the 2-D DIGE gel. To achieve best results from 2-D DIGE gels, ensure the platen and sample press are clean before scanning. The platen is usually cleaned by using Milli-Q water and then dried using tissues, followed by lint-free wipes (Kimberly-Clark, code no. 33370). To remove insoluble material, ethanol can be used instead of water. For stubborn residues, wipe the platen with a tissue dampened with 10% hydrogen peroxide. Rinse well several times with Milli-Q water. It is important to rinse thoroughly at this stage. Failure to do this may leave hydrogen peroxide on the platen which could cause bleaching of fluorescent dyes used in subsequent scans. Dry the platen with a lint-free tissue. The sample press can be clean
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