How do I amplify pBR322 plasmid using chloramphenicol?
Plasmid pBR322 is probably the most well known cloning vector among molecular biologists. A complete review of it’s construction can be found in Bolivar1988. The pUC series of cloning vectors provide a number of additional features including oriented multiple cloning sites. The construction of these can be found in Messing1982 and Vieira1982. The following is the procedure to amplify pUC and pBR based vectors for large scale plasmid preps. The procedure is directly from the Maniatis book (Maniatis1982) descibed under Question 5.
