Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

How do I align my sequence with a single cDNA/mRNA/EST?

April 26, 2017align cDNA EST mrna sequence

0

Posted

How do I align my sequence with a single cDNA/mRNA/EST?

1 Answer

0

Posted

Use the –singleacc option. GrailEXP will look for the specified accession number in the specified list of databases. The accession number must be the same as the one used in the database, e.g. if the accession number in the database is AA393779.1, then just specifying AA393779 will NOT work. You can blame NCBI for this; version number should really be a separate field, as should organism.