How critical is it to add the right amount of 10X Dilution Buffer?
It is extremely critical to add the right amount of 10X Dilution Buffer to the sample as this buffer allows efficient binding of Adenovirus to the Virus-Binding Module. The addition of an incorrect amount of 10X Dilution Buffer will lead to a decrease in the strength of interaction of Adenovirus with the Virus-Binding Module as well as allowing a different spectrum of contaminating proteins to compete with the Adenovirus for binding to the Virus-Binding Module.
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