How concentrated should the RNA be when it is sent to the facility?
The RNA should be resuspended to at least 1.0 µg/µL in RNase-free water. It should have passed the quality control procedures outlined above or, alternatively, you can ask that we assess the quality using the Agilent 2100 Bioanalyzer. The cost for this service is outlined in the fee schedule. Amplification can be done in cases of very low amounts of RNA. Amplifications for two-colour microarrays require a minimum of 50 ng of RNA at a concentration of at least 2.5 ng/µL. Those for one-colour microarrays require at least 200 ng of RNA at a concentration of at least 10 ng/µL. There is an additional charge for amplifications. Please contact us regarding the price.
