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How comparable are the results obtained with the FLIPR Membrane Potential Assay Kit to the standard protocol using DiBAC?

April 26, 2017assay comparable flipr kit membrane obtained potential Protocol results standard

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How comparable are the results obtained with the FLIPR Membrane Potential Assay Kit to the standard protocol using DiBAC?

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Data suggests the Membrane Potential Assay Kit is far superior; the dye is much faster, the signal-to-background is higher, and the throughput can be increased ten-fold. For example, membrane depolarization can be detected with the kit in less than 10 seconds. In addition, data can be obtained using the FLIPR Membrane Potential Assay Kit where equivalent data is unobtainable with the standard protocol, especially small changes in membrane potential.