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How comparable are the results obtained with the FLIPR Calcium Assay Kit to those from standard protocols using the conventional fluo-3 and fluo-4 dyes?

April 26, 2017assay calcium comparable conventional flipr kit obtained protocols results standard

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How comparable are the results obtained with the FLIPR Calcium Assay Kit to those from standard protocols using the conventional fluo-3 and fluo-4 dyes?

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The results obtained with the FLIPR Calcium Assay Kit are highly comparable to those obtained from the conventional assay protocols. The maximum signal obtained with the assay kit is usually equal to or higher than the responses seen with fluo-3 for most cell types and comparable to that achieved with fluo-4.