How come the OST corresponding to my cloned ORF is only partially overlapping that ORF?
The OST displayed in the alignment image of each clone-information page represents the available sequence obtained by sequencing the clone at both ends. In case of long clones the Forward and Reverse tags don’t overlap and provide an only partial confirmation of the predicted gene structure. This is also true when only one of the two sequence tags is of good enough quality to be aligned to it’s ORF. By clicking on the “See OST” link on each clone-information page, we show the complete sequence of each OST that wereceived for that clone (even those OSTs that did not pass the quality treshold to be aligned by Acembly).
