How come DNA is detectable down to 1fM on the SPRimager®II?
You can achieve high-sensitivity detection of specific DNA targets using GWC Technologies’ proprietary AmpliFast™ method of on-chip amplification. The label-free array-based method uses RNA oligo probes, which are degraded in the presence of RNAseH only when target DNA hybridizes to them. Target DNA is not degraded, and amplifies the signal by further hybridizing to remaining RNA probe molecules. Since the method provides real-time data, you can quantify both abundant and rare targets on the same chip.
