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How can you confirm that fluorescence is emitted via GFP-eIF4A binding on the mRNA aptamer rather than random combination of the split GFP-eIF4A?

April 26, 2017aptamer Binding combination confirm emitted fluorescence mrna Split

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How can you confirm that fluorescence is emitted via GFP-eIF4A binding on the mRNA aptamer rather than random combination of the split GFP-eIF4A?

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A. According to research papers, there is very low background fluorescence when the mRNA aptamer is not added into the solution. This means that the probability of random combination of split GFP-eIF4A is extremely low.