How can the tag be detected?
For fast detection we offer Strep-Tactin conjugated to HRP or AP. The Strep-tag HRP Detection Kit [Cat.-no.: 2-1502-000] and the Strep-tag AP Detection Kit [Cat.-no.: 2-1503-000] provide the necessary tools to detect Strep-tag II fusion proteins in Western blots. These ready-to-use systems include all buffers and reagents for blocking, washing and the chromogenic reaction. For high specificity the monoclonal antibody against Strep-tag II can be used [Cat.-no.: 2-1507-001, 2-1509-001]. It is available affinity purified and unlabelled or affinity purified and labelled with HRP. • Which detection procedure (ECL or chromogenic) do you recommend for HRP Strep-Tactin® conjugates? Both, chemiluminescene as well as chromogenic detection, are possible. Please note, that the application protocols for these procedures differ. The protocols are described in the “Western blot protocol” which is also available as pdf on the IBA download/tech info site. • What are the KD-values of the monoclonal anti
