How can the purity of DCs isolated with the Blood Dendritic Cell Isolation Kit II, human be evaluated?
The Blood Dendritic Cell Enumeration Kit II, human is the most convenient way for analysis. It includes pre-mixed, ready-to-use cocktails for DC detection and for isotype control and allows exclusion of dead cells for more reliable results. The Blood Dendritic Cell Enumeration Kit II, human was developed on the basis of unique BDCA markers. It contains the markers CD1c (BDCA-1), CD141 (BDCA-3), and CD303 (BDCA-2) for the characterization of different blood DC subsets. To allow the exclusion of B cells and monocytes from DC analysis, the antibody cocktails contain antibodies against CD19 and CD14, respectively.
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