How can single cells be deposited on the AmpliGrid?
Single cells can be deposited on the AmpliGrid using different techniques such as: • Flow cytometry (see video) • Micromanipulation (use 0.05x PBS to micromanipulate the cells) • Laser microdissection (e.g., PALM system) • Pipetting dilutions Avoid to desposit excessive amounts of buffer when depositing cells as the buffer crystals can lead to bubble formation. Additionally large buffer amounts dramatically decrease efficiency of PCR due to changes in the buffer concentrations. This is particularly important if you hand pipette cells onto the AmpliGrid. Buffer should never exceed 0.5ul (0.05x PBS deposition buffer). 7. If I don’t use all 48 sites in my experiment, can I re-use the unused sites in a future experiment? Yes, one can perform consecutive runs on the AmpliGrid, e.g. use rows A and B today, C and D tomorrow. In this case one has to ensure that no contamination does occur on the non-used spots (by smearing or mispipetting). 8. How can I recover the aqueous phase/PCR solution a
