How can separate treatments be done using ten wells on one microscope slide?
The Teflon masking is a hydrophobic surface between the virtual wells on the slides. Careful, accurate pipetting of the media into the wells results in confinement of the media to the well. Batch processing of the Teflon masked slides by wetting in Coplin jars should be followed by gentle aspiration of the liquid from the Teflon masking area, which greatly facilitates confinement of the media to the wetable surface in the well. Volumes between 25 and 40 microliters per well are easily confined to the well area.
Related Questions
- I have heard of other separate PC programs for merging differently focused microscope images, cheaper too. Why can I use them instead?
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