How can I verify that the silver enhancement reagents are still fine?
Again, there is a simple procedure to check this. It is described in great detail in our Newsletter #4 (please refer to the “Newsletter” section of this web site). In short: you need a nitro-cellulose strip, apply dots from a dilution series of your gold conjugate and incubate the strip with the silver enhancement reagents. The dots should become brown-black. During this period of time the mix of reagents should remain glass clear without any visible presence of silver caused by auto nucleation. The activity of the Silver Enhancement reagent SE-EM for Electron microscopy can be tested by adding 10µl of the diluted ultra small reagent to 100µl of the enhancement mix. The solution should turn yellow in 30-45 minutes.
