How can I use the SureSilencing™ shRNA plasmids for stable transfection and knock down in a cell line that is already resistant to both neomycin and puromycin?
SureSilencing™ shRNA plasmids with other selectable markers are not available at this time, nor do they have convenient restriction sites to reclone the U1-based shRNA expression cassette into another vector expression system. However, you may use a different vector system with another selectable marker (hygromycin), order our SureSilencing shRNA Plasmids with the GFP marker, and perform a co-transfection with these two plasmids in a 10:1 selection marker-to-GFP plasmid molar ratio.
Related Questions
- Can I use fluorescence microscopy to assess shRNA plasmid transfection efficiency in my model cell line of interest, when using a vector expressing a GFP reporter gene?
- How can I use the SureSilencing™ shRNA plasmids for stable transfection and knock down in a cell line that is already resistant to both neomycin and puromycin?
- What cell line does SABiosciences use for quality control testing of the SureSilencing™ shRNA plasmids?
