How can I recover the aqueous phase/PCR solution after the reaction is finished?
There are two possibilities to recover the amplicon: 1. Pipet off the 1µl aqueous phase using a standard 1-10µl pipette (see video). If you would like to have a better visualization of the reaction, please use the AdvaGold dye. 2. Add “liquid”: up to 5µl additional volume can be added after the PCR. This might be gel loading dye as well as any kind of solution which has a higher density than oil. Pipetting off the resulting 6µl is easy and fast.
