How can I perform deuteration reactions in the H-Cube®?
To perform a deuteration reaction it is very important that as much H2O is removed from the system as possible. Step 1: A stainless steel system is essential for this because it has the Purge water function on the service screen. Step2: Drain the water from the reservoir using a syringe. Step 3: Remove the back of the water reservoir with the allan key. Clean and dry the insides with a paper towel. Step 4: Go to the service screen. Press Purge water function and press stop at 10 seconds. Step 5: Replace water reservoir. Add 30 mL of deuterated water. Press Purge Water for 10 seconds. Step 6: Undo water reservoir, dry insides, again and press Purge water for 10 seconds again. Step 7: Replace water reservoir and fill with deuterated water. Press Purge water to refill cell with D2O. Step8: Take a blank cartridge(titanium) and pass an aprotic solvent (a solvent which doesn’t have OH or COOH in the structure) and run the system at Full H2 mode for 10 minutes. Remove blank cartridge. System
