How can I minimize cross-contamination of proteins between the nuclear and cytoplasmic fractions?
Typically, cross-contamination between the nuclear and cytoplasmic fraction is 10% or less when using this kit. Careful pipetting and performing the incubation and centrifugation as indicated in the instructions will minimize cross-contamination. Performing an additional wash of the nuclear pellet with phosphate buffer before adding the NER Reagent will reduce carryover of the cytoplasmic proteins to the nuclear protein fraction.
Related Questions
- Since blastular cells differ in size within the Xenopus embryo, how can nuclear/cytoplasmic ratios regulate transcription?
- How can I minimize cross-contamination of proteins between the nuclear and cytoplasmic fractions?
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