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How can I insure that reaction volume is not lost due to evaporation during thermal cycling?

April 26, 2017cycling evaporation insure Lost REACTION Thermal volume

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How can I insure that reaction volume is not lost due to evaporation during thermal cycling?

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Be sure to carefully and completely seal the qPCR assay plate with fresh optical thin-wall 8-cap strips or adhesive optical film before the plate is placed into your thermal cycler. In addition, refer to your instrument’s user’s manual to determine if the thermal cycler manufacturer recommends the use of a plate compression pad during the run.