How can I improve the efficiency of my SgfI digestion?
We have heard that there can be lot-to-lot variations of the commercially available SgfI enzyme. Several of our scientists have begun to use the “FastDigest® AsiSI (SfaAI) ” enzyme (Fermentas) that has the same recognition sequence. In our hands the double digestions with MluI are complete in a shorter time using this enzyme compared to some but not all SgfI batches.
