How can I get rid of platelets that contaminate the enriched cell fraction?
• most important for users who want to pick tumor cells platelets often stick to these cells • avoid harvesting the whole plasma layer by pouring-off the whole upper phase • selectively harvest interphase with a serological pipette • excessive contamination with platelets can be eliminated by performing a second centrifugation in a 4 to 20% sucrose gradient, tumor cells will be pelleted
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