How can I find the sequence for the cloning vector pBR322 without having to wade through every other record in GenBank that refers to pBR322?
Begin at the initial Entrez Nucleotide database search page. Restrict the search field to Properties and enter “gbdiv syn” into the query box. Pressing the Search button reveals that about 3,100 records have been selected. These belong to the GenBank “Synthetic” division. Into the Add Terms to Query box that now appears, type “pbr322 complete,” and change the search field to Title Word. Pressing the Search button now yields a single sequence, which is that of the pBR322 cloning vector. Follow an analogous procedure for other vector sequences.
Related Questions
- How can I find the sequence for the cloning vector pBR322 without having to wade through every other record in GenBank that refers to pBR322?
- How do I clone my insert into a transfer vector with no viable restriction sites but compatible with the Gateway ® cloning system?
- How do I find out when a sequence record was released to the GenBank public database?
