How can I exclude poor quality data from the mutation report?
You may exclude poor quality data from the report either before or after data analysis. To exclude prior to analysis, go to Process>Options>Output and set the Lane Quality Threshold to 5 for example. The software will then reject lanes with a quality score (signal to noise ratio) below 5 from the statistical mutation frequency calculations. You may also delete lanes with poor quality data after running the analysis by right clicking directly above the electropherogram where it says “Quality.” The mutations in this lane will then be excluded from the overall mutation frequency calculations.
