How can I determine the separation efficiency between the cytoplasmic and nuclear fractions?
Cross-contamination of the nuclear fraction by cytoplasmic proteins can be determined by probing for a protein known to reside only in the cytoplasm, such as a heat shock protein 90 (Hsp90), b-galactosidase or protein kinase C. Determine contamination of the cytoplasmic fraction by probing for Oct-1 or p53 nuclear proteins in the cytoplasmic fraction.
Related Questions
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- How can I determine the separation efficiency between the cytoplasmic and nuclear fractions?
