How can I cut out or re-clone the insert from a Gateway® expression vector?
In case the insert is located in a Destination Vector compatible with the Gateway System, in general the insert cannot be precisely removed using restriction enzymes, as the vector was designed to allow the shuttling (insert transfer) by a site-specific recombination (no multiple cloning site). However, the att sequences contain a BsrGI restriction site which can be used, if short stretches of flanking sequences are acceptable and the enzyme does not cut within your insert sequence.
