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How can I culture for the types of mycobacteria that will not grow on ordinary Middlebrook and Lowenstein Jensen?

April 26, 2017culture grow jensen lowenstein mycobacteria types

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How can I culture for the types of mycobacteria that will not grow on ordinary Middlebrook and Lowenstein Jensen?

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A. Mycobacterium haemophilum can be grown on a Middlebrook 7H11 plate that has an “X-Factor Strip” placed on the surface. This supplies the hemin that this organism requires (ref: Clin. Micro. Newsletter, Vol. 14:11, June 1, 1992).