How can I check if the insert of a particular clone will cover the complete coding sequence?
Please check how the indicated insert sequence (GenBank Accession ID) maps to the sequence you are interested in. Usually, the RefSeq entry is accepted as the official sequence or structure of a transcript, and is chosen by most researchers as a reference. The RefSeq entry for a transcript (Accession ID NM_XXXXXX) can be retrieved from the RefSeq database (NCBI, accessible via EntrezGene). You can easily navigate to a given RefSeq entry of a gene via a hyperlink provided at the top of a respective GenomeCube search result webpage, showing the product list. The corresponding cDNA sequence can also be searched by the Ensembl Genome Browser. (more details) To perform an alignment of the sequences you may use the tool bl2seq from NCBI. To receive the cds annotation in the output, reformat the result (“Formatting options” and checkmark “CDS feature”) to display that annotation.
