How can I calculate the correct rotor speed (RPM) to use for pelleting my cells by centrifugation?
Cells should be pelleted by centrifugation at 180 x g (relative centrifugal force, RCF). The correct rotor speed can be calculated by measuring the maximum radius of your rotor and entering the information into the table found at this website which can be reached via this link: www.eppendorfna.com/support/online-calculator.asp For example, to centrifuge cells at 180 x g (RCF) in a rotor with a radius of 15 cm, you would spin the cells at 1036 rpm.
