How can HotStart simplify my RT-PCR procedure?
A. HotStart storage and reaction tubes have a adhered wax bead on the side of the tube. This lets you carry out RT-PCR in one tube by: 1) adding your RT reagents and a thermostable reverse transcriptase to the tube; 2) melting the wax and letting the RT reaction proceed; and 3) adding your PCR reagents above the wax barrier and placing the tube in a thermocycler. The wax will then melt, giving you a “hot start” with increased yield and specificity. Q.
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- We are sending circulars daily and are exporting the clients data from our register to MultiMailer. How do we simplify this procedure?
- How can HotStart simplify my RT-PCR procedure?
