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How can factor Xa be removed from the reaction mix after cleavage?

April 26, 2017cleavage Factor mix REACTION removed XA

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How can factor Xa be removed from the reaction mix after cleavage?

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Factor Xa can be removed by passing the reaction mix over a small benzamidine-agarose column (GE Healthcare, #17-5143-02). When 50 µg of Factor Xa is passed over a 0.5 ml column, less than 0.2% of the activity flows through.