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How can digital scales be used to calibrate pipettes?

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How can digital scales be used to calibrate pipettes?

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• A: First, you will need several items to begin: pipette, beaker, erlenmeyer flask, thermometer, weigh balance, pipette filler and a pen and paper. Make sure that the pipette and beaker are clean and dry (any residue could adversely effect measurements). Next, place distilled water in the erlenmeyer flask for 15 minutes. After 15 minutes is up, measure the temperature of the water. Determine the mass of the beaker to the nearest tenth of a milligram, using a balance for this step. Next, fill the pipette using the pipette filler with water from the erlenmeyer flask and deposit the water into the beaker. Weigh the beaker again and record the difference in weight from the earlier measurement. Repeat this step three more times. Now, calculate the mean of the four pipette measurements. Next, add 1.06 mg per gram to the mean mass. This must be done to adjust for the buoyancy of air during the weighing. If you are using a digital scale, ignore this step. Now, determine the density of water a

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