How are transcripts that are predicted to be subject to nonsense-mediated decay (NMD) managed?
When RefSeq curators review gene-specific cDNAs, they identify nonsense-mediate decay (NMD) candidates as those with a termination codon at least 50 nucleotides upstream of the last splice junction. Curators review the literature, transcript alignments to the assembled genome, protein homology, and genome conservation during the course of their reviews. Curators make a decision about representing a protein derived from an NMD candidate based on the following guidelines: • If a gene is considered to encode a protein (and there is no evidence to change the locus type to a non-coding option), then curators represent the best supported protein even if it is encoded by a NMD transcript. These RefSeq records use the NM_ and NP_ accession prefix for the transcript and protein, respectively. • If a protein encoded by a gene can be represented using a transcript that is not predicted to be subject to NMD (with NM_ and NP_ accessions), then any additional NMD transcript variant is represented as
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