How are the images scaled by the web site? Why?
By default, pixel values in images are scaled for viewing in the web site. This is done because typical images generated by flourescence miscroscopy are very dim, and would appear black or nearly black to the naked eye. Pixel values, or intensity values, are rescaled for viewing using the following algorithm: The minimum (min) and maximum (max) intensity for the image are found. The intensity for each pixel is then set as: ( ( pixel intensity – min ) / ( max – min ) ) * 65535 (note: this formula presumes 16-bit greyscale images.) This scaling may be toggled on and off when viewing experiments by clicking “Turn Scaling OFF/ON.” Note: Image data downloaded from the YRC PIR is always unscaled.
