How are the hES cells characterized at Cellartis?
Each hES cell line derived at Cellartis is extensively characterized. The characterization includes stem cell marker analysis (SSEA-1, -3, -4; TRA-1-60, -1-81 and Oct-4), and genetic characterization (karyotyping and FISH). Furthermore, telomerase and alkaline phosphatase activities are analyzed and the cells are assayed for in vivo and in vitro differentiation potential. The cell lines at Cellartis have been cultured in vitro for extended periods and endured repeated freezing and thawing cycles, while retaining pluripotency and stable karyotypes. For further information about the characteristics of the cell lines from Cellartis please see Heins et al. (Stem Cells, 2004).
