How are the Caspase activity assays used?
Our Caspase Activity Assays provide a simple and convenient means for assaying protease activity in cell lysates. Upon cleavage of the Caspase-specific peptide substrate, a free reporter molecule can be quantified using a colorimetric or fluorometric microtiter plate reader. Comparison of the signal generated from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase activity. The level of Caspase enzymatic activity is directly proportional to the signal detected.