How are subcellular localization images acquired?
For a detailed description of the methods used, refer to: Aturaliya RN, Fink JL, Davis MJ, Teasdale MS, Hanson KA, Miranda KC, Forrest AR, Grimmond SM, Suzuki H, Kanamori M, Kai C, Kawai J, Carninci P, Hayashizaki Y, Teasdale RD (2006). Subcellular localization of mammalian type II membrane proteins. Traffic. 7(5):613-25. PubMed Abstract Epifluorescent microscopy is used for first-pass localization. Co-localizations are performed using confocal microscopy. A collection of images is chosen based on analysis of the observed expression patterns with each construct. One of these images is chosen to represent the construct. Images are captured at 60X or 100X magnification.
