How are pollen and mold counted?
The collection tape is removed from the sampling device and brought to the laboratory. Here it is stained and prepared for analysis. The sample can then be magnified 400 times to count the pollen grains. For some mold spores, the sample must be magnified 1000 times to be seen and counted. Using the exposure time, the volume of air sampled, and the number of pollen grains or mold spores counted, calculations can be made to determine the number of particles per cubic meter of air sampled. This is the number reported by the laboratory.