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How are DNA fragments used to make recombinant dna?

April 26, 2017dna fragments recombinant Used

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How are DNA fragments used to make recombinant dna?

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Two DNA fragments with protruding ends complementary to each other are joined using a Ligase and blunt ended DNA fragments are joined by means of adding additional complementary sequences at their ends with the help of linkers, adapters or by using a terminal transferase for Homopolymer Tailing.