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How and when are assemblies built?

Assemblies built
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How and when are assemblies built?

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Assemblies are built when the underlying datasets change. We currently aim at one to two releases per year. For the assembly process we use the sequence from mapped and finished clones as a scaffold. The remaining gaps get filled with sequence from whole genome shotgun supercontigs, produced by assembling reads with Phusion. Markers, BAC end sequences and cDNAs are used as anchors to merge clone and contig sequence. This results in three categories of contigs. Firstly, contigs consisting of finished BAC sequences and gaps in between filled with WGS sequence, placed onto a chromosome. Secondly, the same but without any chromosome placement. These two types of contigs will be named Zv8_scaffold. Thirdly, some WGS contigs can’t be tied to the finished clone sequences at all, these will go into the assembly as Zv8_NA. After all this, the assembly is released to the public.

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Assemblies are built when the underlying datasets change. We currently aim at a release every one or two years. For the assembly process we use the sequence from mapped and finished clones as a starting point. The remaining gaps get filled with sequence from whole genome shotgun supercontigs, as described in FAQ number 1 from the “Facts” section. Markers and cDNAs are used as anchors to merge clone and WGS contig sequence. This results in three categories of contigs. Firstly, contigs consisting of finished BAC sequences and gaps in between filled with WGS sequence, placed onto a chromosome. Secondly, the same but without any chromosome placement. These two types of contigs will be named Zv9_scaffold. Thirdly, some WGS contigs can’t be tied to the finished clone sequences at all, these will go into the assembly as Zv9_NA. After all this, the assembly is released to the public. For further details about identifiers in the assembly, see FAQ number 7 about searching the San

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