Have you compared the efficiency of your technology in the isolation of viable cancer cells with magnetic cell separation and Ficoll-Paque density centrifugation?
The specificity and sensitivity of Vita-Assay™ and Vita-Cap™ have been determined using model experiments, in which 1 to 1,000 tumor cells are spiked into one mL of normal blood. We also included a quality control of our products by directly comparing the capture efficiency of viable cancer cells from whole blood with current cell fractionation methods (antibody-magnetic cell separation and density centrifugation). As available antibodies are directed against epithelial cells, we also use commonly used antibodies against epithelial cells to detect cells recovered from our products. Our products generate highly enriched epithelial cell population that enables greater than 25 folds of detection sensitivity of epithelial cells in blood than other methods.
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